使用MagStart-Amine、MagStart-HILIC和MagStart-SAX三種磁珠對蛋白的酶解液除雜的簡易流程
|
Conditions |
MagStart-Amine (PAC) |
|
Bind |
50%~80% ACN （or EtOH）pH=8 |
|
Wash |
80% EtOH and 95% ACN |
|
Digest |
LysC / Trp （1-4 hour） |
|
Elute |
H2O or 0.5% TFA or DMSO |
|
Range |
1 μg~200 μg |
|
Extration |
SDS，NP40，Tween， SDC |
|
Clean-up of |
Proteins |
|
Fractionation |
NA |
|
Top-down |
NA |
胺基磁珠 MagStart-Amine——高結合量蛋白質組學前處理-生物磁珠專家 (purimagbead.com)
|
Conditions |
MagStart-HILIC |
|
Bind |
15 CAN，100 mM NH4Ac，pH=4.5 |
|
Wash |
95% ACN |
|
Digest |
LysC / Trp （1-4 hour） |
|
Elute |
1% TFA |
|
Range |
5 μg~0.5 mg |
|
Extration |
SDS，NP40，Tween， SDC |
|
Clean-up of |
Proteins，Peptides & Glycans |
|
Fractionation |
NA |
|
Top-down |
NA |
HILIC磁珠 MagStart-HILIC——高結合量蛋白質組學前處理-生物磁珠專家 (purimagbead.com)
|
Conditions |
MagStart-SAX |
|
Bind |
100 mM Borate pH=10 |
|
Wash |
10 mM Borate pH=10 |
|
Digest |
LysC / Trp （1-4 hour） |
|
Elute |
1% TFA （frac pH10→2） |
|
Range |
20 μg~5 mg |
|
Extration |
Urea |
|
Clean-up of |
Proteins & Peptides |
|
Fractionation |
Salt or pH Gradient |
|
Top-down |
Salt or pH Gradient |
SAX磁珠 MagStart-SAX——高結合量蛋白質組學前處理-生物磁珠專家 (purimagbead.com)
- 上一篇：國家生物信息中心和武漢大學聯合在Anal. Chem. 發文 2024/5/18
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