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客戶定制20微米磁珠研究微流控操控磁珠在權威分析化學期刊《 Analytical Chemistry》發表論文

2023-11-8 21:46:17點擊:


Fully Integrated Microfluidic Device for Magnetic Bead Manipulation to Assist Rapid Reaction and Cleaning
Zhen Yang, Miaoning Ren, Ya Li, Mingxing Zhou, Jingyi Peng, Si Lin, Kang Du, and Xian Huang*
Cite this: Anal. Chem. 2023, 95, 40, 14934–14943
Publication Date:September 26, 2023
https://doi.org/10.1021/acs.analchem.3c02285


Abstract


Methods to manipulate magnetic beads are essential factors to determine the efficiency and dimension of an in vitro diagnostic system. Currently, using movable permanent magnets and planar electromagnets is still the major approach to achieve magnetic bead control, causing significant constraint in the miniaturization of in vitro diagnostic systems. Here, we propose techniques to construct a fully integrated microfluidic device that can conduct automatic magnetic bead manipulation as well as rapid chemical reaction and cleaning in a minimized dimension similar to a USB disk. The device combines the precision control of multiple electromagnetic coils with the compactness of microfluidic channels, leading to one of the smallest automatic magnetic bead manipulation systems that can complete several major magnetic bead-based operation steps such as sample injection, reaction, cleaning, and collection. The influencing factors such as coil driving parameters, surface treatment of the microchannels, and properties of magnetic particles have also been investigated to optimize the device performance. The device can drive mixtures of Fe3O4 microparticles and polymer magnetic beads (PMBs) with a weight ratio of 1:1 at a maximum speed of 0.5 cm·s–1 and reduce the time for DNA binding and dissociation reactions from 20 min to only 48 s. This device has significantly advanced the conventional manipulation methods of magnetic beads and has demonstrated the possibility to construct an automatic and ultraminiaturized in vitro diagnostic system that may facilitate portable or even wearable chemical analysis.


操縱磁珠的方法是決定體外診斷系統的效率和尺寸的重要因素。目前,使用可移動永磁體和平面電磁鐵仍然是實現磁珠控制的主要方法,這對體外診斷系統的小型化造成了重大制約。在這里,我們提出了構建一種完全集成的微流體設備的技術,該設備可以進行自動磁珠操作,并以類似于U盤的最小尺寸進行快速化學反應和清潔。該設備將多個電磁線圈的精確控制與微流體通道的緊湊性相結合,成為最小的自動磁珠操作系統之一,可以完成幾個主要的基于磁珠的操作步驟,如樣品注入、反應、清潔和收集。還研究了線圈驅動參數、微通道的表面處理和磁性粒子的性質等影響因素,以優化器件性能。該設備可以以0.5 cm·s–1的最大速度驅動重量比為1:1的Fe3O4微粒和聚合物磁珠(PMBs)的混合物,并將DNA結合和解離反應的時間從20分鐘縮短到僅48秒。該設備顯著改進了磁珠的傳統操作方法,并證明了構建自動超小型體外診斷系統的可能性,該系統可以促進便攜式甚至可穿戴的化學分析。