不让我穿衣服随时吃奶,欧美激情综合亚洲一二区,xxxxxbbb,色干综合,欧美a色,2021国产精品久久久久精品

Conditions

MagStart-Amine (PAC)

Bind

50%~80% ACN （or EtOH）pH=8

Wash

80% EtOH and 95% ACN

Digest

LysC / Trp （1-4 hour）

Elute

H₂O or 0.5% TFA or DMSO

Range

1 μg~200 μg

Extration

SDS，NP40，Tween， SDC

Clean-up of

Proteins

Fractionation

NA

Top-down

NA

Conditions

MagStart-HILIC

Bind

15 CAN，100 mM NH₄Ac，pH=4.5

Wash

95% ACN

Digest

LysC / Trp （1-4 hour）

Elute

1% TFA

Range

5 μg~0.5 mg

Extration

SDS，NP40，Tween， SDC

Clean-up of

Proteins，Peptides & Glycans

Fractionation

NA

Top-down

NA

Conditions

MagStart-SAX

Bind

100 mM Borate pH=10

Wash

10 mM Borate pH=10

Digest

LysC / Trp （1-4 hour）

Elute

1% TFA （frac pH10→2）

Range

20 μg~5 mg

Extration

Urea

Clean-up of

Proteins & Peptides

Fractionation

Salt or pH Gradient

Top-down

Salt or pH Gradient